By capillarity. The trough has small perforations that allow the root to pass through, making the root development visible. The seeds are placed in the trough located at the top of the pouch and the water is deposited at the bottom of the pouch. The water moves upwards via the paper wick. Once it reaches the trough, it will moisten the seed.
It varies. Some seeds will consume the water faster than other seeds. The best criterion is to keep the wick moist at all times. When the wick starts to dry up, add 10 ml. of water.
Yes, the pouch can be autoclaved. The pouch is designed to withstand that process. However, the polypap stand will not withstand autoclave temperatures.